Create a black-reference (DSNU)

1. Switch off the illumination.

2. Cover the lens with a black or dark piece of cardboard or plastic.
   No light may reach the sensor.

3. In the menu bar navigate to Tools → Calibration.

4. Click Generate DSNU Reference.

The Generate DSNU Reference wizard opens.

5. Click Acquire image to generate the DSNU directly from the camera
   or
   click Load from Disk to load an image from the hard drive.
   

   NOTE: Load from Disk

   Make sure that the image has been taken with active image calibration mode by using the wizard.

The raw image and the line plot of the image is displayed. 

Send the calculated DSNU to the camera: 

6. Select LUT 1 or LUT 2.

7. Click Send data to camera.

8. In the Configuration window navigate to Camera Image Calibration → DSNU/PRNU.

9. Make sure that DSNU is enabled.